Karen

Karen is the wife and henchman of Yes-Man and a minor antagonist in Miss Carol Anne vs. the Twerk Team. Tosh usually nicknames her "stink butt" to make fun of her, which usually irritates her. She also works as a teacher in Twerkvile Highschool and an aid of an annoying kid named Nicolas, as shown in "Karen Gets A Job." In the episode "Been There, Nicolas That," she teamed up with Miss Carol Anne, along with the help of her husband Yes-Man, to bust Nicolas and get him expelled from school, which ended up getting her fired, along with Miss Carol Anne and Yes-Man being arrested by The Twerk Team (who were disguised as police officers).

She is also an inventor. Her inventions are usually used by Yes-Man.

In the episode "The Haunting Hour," she hired The Flaunt Boys to steal the Booty Gem after The Twerk Team and Miss Carol Anne dropped it in a corn maze. In the episode "Too Many Tinas," she created a cloning machine which Yes-Man used to clone Tina.

She is voiced by Alex Borstein.